| Autor: |
Serebrovskaia EO, Stremovskiĭ OA, Chudakov DM, Luk'ianov KA, Deev SM |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2011 Jan-Feb; Vol. 37 (1), pp. 137-44. |
| DOI: |
10.1134/s1068162011010134 |
| Abstrakt: |
Photosensitizer-antibody conjugates are successfully used for targeted elimination of cancer cells bearing specific membrane markers. This method is known as photoimmunotherapy. However, chemical conjugation of photosensitizer and antibody poses a number of complications such as low reproducibility, aggregation and unconjugated photosensitizer impurities. Here we report a fully genetically encoded photoimmunosensitizer, consisting of an anti-HER2/neu miniantibody 4D5scFv and a phototoxic fluorescent protein KillerRed. Both domains in this photoimmunosensitizer retained their functional qualities - high affinity for HER2/neu antigen and phototoxicity respectively. 4D5scFv-KillerRed fusion protein showed high specificity for HER2/neu-over-expressing cells and effectively lowered their viability upon illumination. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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