| Autor: |
Demina IA, Serebriakova MV, Ladygina VG, Galiamina MA, Zhukova NA, Alekseev DG, Fisunov GIu, Govorun VM |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2011 Jan-Feb; Vol. 37 (1), pp. 70-80. |
| DOI: |
10.1134/s1068162011010067 |
| Abstrakt: |
Saturating proteome identification and the study of post-translational protein modifications of Acholeplasma laidlawii using combination of single- and two-dimension gel electrophoresis followed by mass-spectrometry analysis have been carried out. Results were compared to the earlier identified proteome of Mycoplasma gallisepticum. It was found that M. gallisepticum and A. laidlawii express 61 and 58% of the annotated ORFs respectively. All subunits of DNA-polymerase III were identified during our study which indicates that our methods can detect single copies of a given protein per cell. Metabolic pathways of the respective mycoplasmas were compared further in this work. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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