Identification of anti-inflammatory targets for Huntington's disease using a brain slice-based screening assay.

Autor: Reinhart PH; Discovery Neuroscience, Wyeth Research, Princeton, NJ 08543, USA., Kaltenbach LS, Essrich C, Dunn DE, Eudailey JA, DeMarco CT, Turmel GJ, Whaley JC, Wood A, Cho S, Lo DC
Jazyk: angličtina
Zdroj: Neurobiology of disease [Neurobiol Dis] 2011 Jul; Vol. 43 (1), pp. 248-56. Date of Electronic Publication: 2011 Mar 31.
DOI: 10.1016/j.nbd.2011.03.017
Abstrakt: Huntington's disease (HD) is a late-onset, neurodegenerative disease for which there are currently no cures nor disease-modifying treatments. Here we report the identification of several potential anti-inflammatory targets for HD using an ex vivo model of HD that involves the acute transfection of human mutant huntingtin-based constructs into rat brain slices. This model recapitulates key components of the human disease, including the formation of intracellular huntingtin protein (HTT)-containing inclusions and the progressive neurodegeneration of striatal neurons-both occurring within the native tissue context of these neurons. Using this "high-throughput biology" screening platform, we conducted a hypothesis-neutral screen of a collection of drug-like compounds which identified several anti-inflammatory targets that provided neuroprotection against HTT fragment-induced neurodegeneration. The nature of these targets provide further support for non-cell autonomous mechanisms mediating significant aspects of neuropathogenesis induced by mutant HTT fragment proteins.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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