Transient micro-elastography: A novel non-invasive approach to measure liver stiffness in mice.

Autor:	Bastard C; Echosens, Research and Development Department, Paris, France. cecile.bastard@echosens.com, Bosisio MR, Chabert M, Kalopissis AD, Mahrouf-Yorgov M, Gilgenkrantz H, Mueller S, Sandrin L
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2011 Feb 28; Vol. 17 (8), pp. 968-75.
DOI:	10.3748/wjg.v17.i8.968
Abstrakt:	Aim: To develop and validate a transient micro-elastography device to measure liver stiffness (LS) in mice. Methods: A novel transient micro-elastography (TME) device, dedicated to LS measurements in mice with a range of measurement from 1-170 kPa, was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston. The novel probe was validated in a classical fibrosis model (CCl(4)) and in a transgenic murine model of systemic amyloidosis. Results: TME could be successfully performed in control mice below the xiphoid cartilage, with a mean LS of 4.4 ± 1.3 kPa, a mean success rate of 88%, and an excellent intra-observer agreement (0.98). Treatment with CCl(4) over seven weeks drastically increased LS as compared to controls (18.2 ± 3.7 kPa vs 3.6 ± 1.2 kPa). Moreover, fibrosis stage was highly correlated with LS (Spearman coefficient = 0.88, P < 0.01). In the amyloidosis model, much higher LS values were obtained, reaching maximum values of > 150 kPa. LS significantly correlated with the amyloidosis index (0.93, P < 0.0001) and the plasma concentration of mutant hapoA-II (0.62, P < 0.005). Conclusion: Here, we have established the first non-invasive approach to measure LS in mice, and have successfully validated it in two murine models of high LS.
Databáze:	MEDLINE
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