Housekeeping gene expression in myogenic cell cultures from neurodegeneration and denervation animal models.

Autor: Manzano R; LAGENBIO-I3A, Instituto Aragonés de Ciencias de Salud, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain., Toivonen JM, Calvo AC, Muñoz MJ, Zaragoza P, Osta R
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Apr 22; Vol. 407 (4), pp. 758-63. Date of Electronic Publication: 2011 Mar 31.
DOI: 10.1016/j.bbrc.2011.03.096
Abstrakt: Reliability and accuracy of real-time quantitative PCR results depend on the use of housekeeping genes which must be constitutively expressed thorough the samples of the study. In the present work, we tested the expression stability of six candidate housekeeping genes (Actb, Rn18s, Gapdh, Hprt1, Sdha and B2m) considering sex, age, muscle-type and neurodegeneration or denervation status in mouse muscle satellite cells. Their expression varied under all variables tested; therefore the ranking of the most suitable genes for the normalization is modified depending on the factors included in the analysis, especially the age of the donor. Moreover, we describe the unsuitability of Rn18s in analysis comprising samples of different ages. On the other hand, we demonstrate that the use of the two best genes in each case is enough to obtain a reliable normalization factor. In this work, we give a broad information of the best housekeeping genes in mouse myogenic cells depending on the variables included in the experimental design.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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