Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain.
| Autor: | Noens EE; European Molecular Biology Laboratory (EMBL), Hamburg Outstation, c/o DESY, Building 25a, Notkestrasse 85, 22603 Hamburg, Germany. e.noens@embl-hamburg.de, Williams C, Anandhakrishnan M, Poulsen C, Ehebauer MT, Wilmanns M |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | BMC biotechnology [BMC Biotechnol] 2011 Mar 25; Vol. 11, pp. 27. Date of Electronic Publication: 2011 Mar 25. |
| DOI: | 10.1186/1472-6750-11-27 |
| Abstrakt: | Background: The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagged recombinant proteins as a major contaminant when using this expression system. This is likely due to a histidine-rich C-terminus in GroEL1. Results: In order to improve purification efficiency and yield of polyhistidine-tagged mycobacterial target proteins, we created a mutant version of GroEL1 by removing the coding sequence for the histidine-rich C-terminus, termed GroEL1ΔC. GroEL1ΔC, which is a functional protein, is no longer able to bind nickel affinity beads. Using a selection of challenging test proteins, we show that GroEL1ΔC is no longer present in protein samples purified from the groEL1ΔC expression strain and demonstrate the feasibility and advantages of purifying and characterising proteins produced using this strain. Conclusions: This novel Mycobacterium smegmatis expression strain allows efficient expression and purification of mycobacterial proteins while concomitantly removing the troublesome contaminant GroEL1 and consequently increasing the speed and efficiency of protein purification. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|