| Autor: |
Givens KT; Jules Stein Eye Institute, UCLA School of Medicine 90024-7004., Lee DA, Rothschiller J, Kitada S, Larian B, Cortes A |
| Jazyk: |
angličtina |
| Zdroj: |
Current eye research [Curr Eye Res] 1990 Jun; Vol. 9 (6), pp. 599-606. |
| DOI: |
10.3109/02713689008999599 |
| Abstrakt: |
Utilizing a rapid colorimetric assay that relates cell number to cytoplasmic hexosaminidase activity, we conducted drug-induced cytotoxicity experiments on human ocular fibroblasts cultured from Tenon's capsule specimens. The effects of two different agents--dimethyl sulfoxide (DMSO) or mitomycin C--on the proliferation of human ocular fibroblasts were studied. Simultaneously the sensitivity of this technique was compared to electronic cell counting with a Coulter counter, a conventional means of quantifying proliferation. Known numbers of cells were exposed to varied concentrations of either DMSO or mitomycin C for 11 days. Cell attachment was quantified after 24 hours, and proliferation was quantified periodically thereafter over the remaining 10 days. Colorimetric data contained a similar or smaller amount of random error than corresponding Coulter values. Both assays identified statistically significant antiproliferative effects and inhibitory effects on cell attachment at higher drug doses; however, Coulter counting alone detected many additional significant effects among lower-dose DMSO and mitomycin treatment groups. Although the hexosaminidase assay displayed lower sensitivity than Coulter counting, it may still be useful to rapidly screen new compounds for strong antifibroblastic effects. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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