| Autor: |
Utkina LL, Zhabon EO, Slavokhotova AA, Rogozhin EA, Shiian AN, Grishin EV, Egorov TsA, Odintsova TI, Pukhal'skiĭ VA |
| Jazyk: |
ruština |
| Zdroj: |
Genetika [Genetika] 2010 Dec; Vol. 46 (12), pp. 1645-51. |
| Abstrakt: |
A novel antifungal peptide, LAMP-Ia, was isolated from sand-elymus (Leymus arenarius) seeds. Expression of a synthetic gene encoding this peptide in Escherichia coli cells was obtained. The target peptide was expressed as a fusion with thioredoxin. Identity of the recombinant peptide to native LAMP-Ia was confirmed by chromatography, mass spectrometry, and amino acid sequencing. LAMP-Ia displayed a high inhibitory activity in respect of a number of phytopathogenic fungi in in vitro assays, which opens up possibilities for the gene encoding it to be used for genetic transformation of plants and for engineering pathogen-resistant crops. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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