[The absorption-elution technique with the use of highly active proteases: a novel technology for the detection of Rhesus D antigen in bloodstains].

Autor: Kondratova IV, Gusarov AA
Jazyk: ruština
Zdroj: Sudebno-meditsinskaia ekspertiza [Sud Med Ekspert] 2010 Nov-Dec; Vol. 53 (6), pp. 24-6.
Abstrakt: The proposed method for the specific detection of Rhesus D antigen in 6 month old bloodstains is based on the use of modified immunoglobulins (anti-rhesus D peptides) in the absorption-elution reaction (AER). These peptides were prepared by an original method using polyclonal anti-rhesus D antibodies and standard erythrocytes preliminarily treated with a highly active protease. Specific identification of D antigen of the Rhesus system obviates the problem of poor AER specificity and thereby significantly improves the quality of forensic biological expertise.
Databáze: MEDLINE