Enzyme immobilization by entrapment.

Autor: Trevan MD; Biological Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, UK.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 1988; Vol. 3, pp. 491-4.
DOI: 10.1385/0-89603-126-8:491
Abstrakt: Entrapment methods of immobilization are mostly used in cell immobilization procedures, but have found some application with enzymes, particularly when the enzyme is essentially a dead cell or crude homogenate(1). In theory the entrapped enzyme is not attached to the polymer; its free diffusion is merely restrained. In practice, however, some or all of the enzyme may be an integral part of the polymer matrix; for example, chain termination in polyacrylamide gel formation may occur onto the enzyme (see also ref. 2).
Databáze: MEDLINE