Autor: |
Trevan MD; Biological Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, UK. |
Jazyk: |
angličtina |
Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 1988; Vol. 3, pp. 491-4. |
DOI: |
10.1385/0-89603-126-8:491 |
Abstrakt: |
Entrapment methods of immobilization are mostly used in cell immobilization procedures, but have found some application with enzymes, particularly when the enzyme is essentially a dead cell or crude homogenate(1). In theory the entrapped enzyme is not attached to the polymer; its free diffusion is merely restrained. In practice, however, some or all of the enzyme may be an integral part of the polymer matrix; for example, chain termination in polyacrylamide gel formation may occur onto the enzyme (see also ref. 2). |
Databáze: |
MEDLINE |
Externí odkaz: |
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