Performance and suitability of polymerase chain reaction for early detection of bacteria in platelet concentrates.

Autor: Rood IG; Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands., Pettersson A, Savelkoul PH, de Korte D
Jazyk: angličtina
Zdroj: Transfusion [Transfusion] 2011 Sep; Vol. 51 (9), pp. 2006-11. Date of Electronic Publication: 2011 Mar 10.
DOI: 10.1111/j.1537-2995.2011.03090.x
Abstrakt: Background: In this study the applicability of a 16S rRNA real-time reverse transcriptase polymerase chain reaction (RT-PCR) and a Staphylococcus genus-specific PCR for screening of bacterial contamination in platelet concentrates (PCs) was determined.
Study Design and Methods: A total of 336 sample bags, from PCs that were routinely tested in the BacT/ALERT (bioMérieux), were collected and frozen until testing by the PCR assays. Based on the BacT/ALERT results, 107 PCs were positive and 229 were negative for bacterial growth.
Results: The analytical sensitivity of the 16S rRNA real-time RT-PCR ranged from 5 to 40 colony-forming units (CFUs)/mL. The PCR detected five positive samples, four of which were also positive in the BacT/ALERT. The sensitivity of the test was 3.8%, and the specificity was 99.5%. The analytical sensitivity of the Staphylococcus genus-specific PCR ranged from 5 to 15 CFUs/mL. Thirty-nine units that were BacT/ALERT positive for staphylococci were tested with this PCR. Six samples were positive with the PCR, five of which were also BacT/ALERT positive. The sensitivity of the Staphylococcus genus-specific PCR was 12.8%, and the specificity was 98.8%.
Conclusion: Despite the rapid availability of results compared to the BacT/ALERT, the analytical sensitivity of a generic or specific PCR assay is not high enough to be an alternative for the BacT/ALERT when PCs are screened on the day of production.
(© 2011 American Association of Blood Banks.)
Databáze: MEDLINE
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