| Autor: |
Bannister SC; CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia. stephanie.bannister@univie.ac.at, Smith CA, Roeszler KN, Doran TJ, Sinclair AH, Tizard ML |
| Jazyk: |
angličtina |
| Zdroj: |
Biology of reproduction [Biol Reprod] 2011 Jul; Vol. 85 (1), pp. 22-30. Date of Electronic Publication: 2011 Mar 09. |
| DOI: |
10.1095/biolreprod.110.088476 |
| Abstrakt: |
Tissue-specific patterns of microRNA (miRNA) expression contribute to organogenesis during embryonic development. Using the embryonic chicken gonads as a model for vertebrate gonadogenesis, we previously reported that miRNAs are expressed in a sexually dimorphic manner during gonadal sex differentiation. Being male biased, we hypothesised that up-regulation of microRNA 202* (MIR202*) is characteristic of testicular differentiation. To address this hypothesis, we used estrogen modulation to induce gonadal sex reversal in embryonic chicken gonads and analyzed changes in MIR202* expression. In ovo injection of estradiol-17beta at Embryonic Day 4.5 (E4.5) caused feminization of male gonads at E9.5 and reduced MIR202* expression to female levels. Female gonads treated at E3.5 with an aromatase inhibitor, which blocks estrogen synthesis, were masculinized by E9.5, and MIR202* expression was increased. Reduced MIR202* expression correlated with reduced expression of the testis-associated genes DMRT1 and SOX9, and up-regulation of ovary-associated genes FOXL2 and CYP19A1 (aromatase). Increased MIR202* expression correlated with down-regulation of FOXL2 and aromatase and up-regulation of DMRT1 and SOX9. These results confirm that up-regulation of MIR202* coincides with testicular differentiation in embryonic chicken gonads. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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