Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes.

Autor: Francisco CL; UNESP, Univ Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Produção Animal, P. O. Box 560, 18618-000 Botucatu, São Paulo, Brazil. carolinelimafrancisco@gmail.com, Jorge AM, Dal-Pai-Silva M, Carani FR, Cabeço LC, Silva SR
Jazyk: angličtina
Zdroj: Meat science [Meat Sci] 2011 Jul; Vol. 88 (3), pp. 535-41. Date of Electronic Publication: 2011 Feb 15.
DOI: 10.1016/j.meatsci.2011.02.007
Abstrakt: This study aimed to evaluate myosin heavy chain (MyHC) isoform expression and muscle fiber types of Longissimus dorsi (LD) and Semitendinosus (ST) in Mediterranean buffaloes and possible fibers muscles modulation according to different slaughter weights. The presence of MyHC IIb isoforms was not found. Only three isoforms of MyHC (IIa, IIx/d and I) were observed and their percentages did not vary significantly among slaughter weights. The confirmation of the presence of hybrid muscles fibers (IIA/X) in LD and ST muscles necessitated classifying the fiber types into fast and slow according to their contractile activity, by m-ATPase assay. For both muscles, the muscle fiber frequency was higher for fast than for slow fibers in all weight groups. There was a difference (P<0.05) in the frequency of LD and ST muscle fiber types according to slaughter weights, which demonstrate that the slaughter weight influences the profile of muscle fibers from buffaloes.
(Copyright © 2011 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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