| Autor: |
Del Carratore R; Institute of Clinical Physiology, National Research Council (CNR), via Moruzzi 1, Pisa, Italy. rdc@ifc.cnr.it, Gervasi PG, Contini MP, Beffy P, Maserti BE, Giovannetti G, Brondolo A, Longo V |
| Jazyk: |
angličtina |
| Zdroj: |
Biotechnology letters [Biotechnol Lett] 2011 Jun; Vol. 33 (6), pp. 1201-6. Date of Electronic Publication: 2011 Feb 16. |
| DOI: |
10.1007/s10529-011-0557-0 |
| Abstrakt: |
n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the ω-hydroxylase of lauric acid, which is a marker for ω-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink