Overexpression of myosin-IIB in the brain of a rat model of streptozotocin-induced diabetes.

Autor: Calábria LK; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902, Uberlândia-MG, Brazil., da Cruz GC, Nascimento R, Carvalho WJ, de Gouveia NM, Alves FV, Furtado FB, Ishikawa-Ankerhold HC, de Sousa MV, Goulart LR, Espindola FS
Jazyk: angličtina
Zdroj: Journal of the neurological sciences [J Neurol Sci] 2011 Apr 15; Vol. 303 (1-2), pp. 43-9.
DOI: 10.1016/j.jns.2011.01.017
Abstrakt: The Ca(2+)/calmodulin complex interacts with and regulates various enzymes and target proteins known as calmodulin-binding proteins (CaMBPs). This group of proteins includes molecular motors such as myosins. In this study, we show that non-muscle myosin-IIB is overexpressed in the brains of diabetic rats. We isolated CaMBPs from the brains of non-diabetic rats and rats with streptozotocin-induced diabetes and purified them by immobilized-calmodulin affinity chromatography. The proteins were eluted with EGTA and urea, separated by SDS-PAGE, digested and submitted to peptide mass fingerprinting analysis. Thirteen intense bands were found in both types of brains, two were found exclusively in non-diabetic brains and four were found exclusively in diabetic brains. A large fraction of the eluted proteins contained putative IQ motifs or calmodulin-binding sites. The results of the myosin-IIB affinity chromatography elution, western blot and RT-PCR analyses suggest that myosin-IIB protein and mRNA are expressed at high levels in diabetic brains. This is the first study that has demonstrated differential expression of CaMBPs in diabetic and non-diabetic brain tissue through a comparative proteomic analysis, and it opens up a new approach to studying the relationship between the expression of myosins in the brain, hyperglycemia and intracellular calcium regulation.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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