| Autor: |
Höög JL; European Molecular Biology Laboratories, Cell Biology and Biophysics Program, D-69117 Heidelberg, Germany. johanna.hoog@path.ox.ac.uk, Huisman SM, Sebö-Lemke Z, Sandblad L, McIntosh JR, Antony C, Brunner D |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of cell science [J Cell Sci] 2011 Mar 01; Vol. 124 (Pt 5), pp. 693-8. Date of Electronic Publication: 2011 Feb 08. |
| DOI: |
10.1242/jcs.072967 |
| Abstrakt: |
Microtubules (MTs) exhibit dynamic instability, alternating between phases of growth and shortening, mostly at their uncapped plus ends. Based on results from cryo-electron microscopy it was proposed that growing MTs display mainly curved sheets and blunt ends; during depolymerisation curled 'ramshorns' predominate. Observations of MTs in mitotic cells have suggested that the situation in vivo differs from that in vitro, but so far, a clear comparison between in vivo and in vitro results has not been possible because MT end structures could not be correlated directly with the dynamic state of that particular MT. Here we combine light microscopy and electron tomography (ET) to show that growing MT plus ends in the fission yeast Schizosaccharomyces pombe display predominantly a flared morphology. This indicates that MT polymerisation in vivo and in vitro can follow different paths. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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