Search for appropriate experimental methods to create stable hind-limb ischemia in mouse.

Autor:	Goto T; Department of Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan. gotoh-ta@is.icc.u-tokai.ac.jp, Fukuyama N, Aki A, Kanabuchi K, Kimura K, Taira H, Tanaka E, Wakana N, Mori H, Inoue H
Jazyk:	angličtina
Zdroj:	The Tokai journal of experimental and clinical medicine [Tokai J Exp Clin Med] 2006 Sep 20; Vol. 31 (3), pp. 128-32. Date of Electronic Publication: 2006 Sep 20.
Abstrakt:	Objective: Stable animal models for refractory peripheral arterial disease are not established. A standardized animal model of hind-limb ischemia is required upon searching effective treatment for this condition. The aim of the study is to verify previously used hind-limb ischemia models to find a standard method. Methods: Using Balb/ca mice six various methods of inducing hind-limb ischemia were applied and two weeks after operation degree of ischemic damage were examined. Six methods include V group, A group, AV group, A-strip group, AV-strip group and Prox-A group (refer the text). Results: Degree of ischemia was evaluated macroscopically by judging toes, foot, knee, and total hind-limb necrosis. We found that severity of damage was markedly different among different methods. Furthermore the severity of necrosis was not uniform even in the same method group. Conclusions: The A-strip group in which the femoral artery from the bifurcation of the deep femoral artery to the saphenous artery was stripped appears to be suitable as a stable severe ischemia model. The A group in which the femoral artery were cut just below the bifurcation of the deep femoral artery appears to be suitable as a chronic mild ischemia model.
Databáze:	MEDLINE
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