| Autor: |
Mendonça-Franqueiro Ede P; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Alves-Paiva Rde M, Sartim MA, Callejon DR, Paiva HH, Antonucci GA, Rosa JC, Cintra AC, Franco JJ, Arantes EC, Dias-Baruffi M, Sampaio SV |
| Jazyk: |
angličtina |
| Zdroj: |
Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2011 Mar; Vol. 43 (3), pp. 181-92. Date of Electronic Publication: 2011 Feb 04. |
| DOI: |
10.1093/abbs/gmr003 |
| Abstrakt: |
Snake venom lectins have been studied in regard to their chemical structure and biological functions. However, little is known about lectins isolated from Bothrops atrox snake venom. We report here the isolation and partial functional and biochemical characterization of an acidic glycan-binding protein called galatrox from this venom. This lectin was purified by affinity chromatography using a lactosyl-sepharose column, and its homogeneity and molecular mass were evaluated by high-performance liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. The purified galatrox was homogeneous and characterized as an acidic protein (pI 5.2) with a monomeric and dimeric molecular mass of 16.2 and 32.5 kDa, respectively. Alignment of N-terminal and internal amino acid sequences of galatrox indicated that this protein exhibits high homology to other C-type snake venom lectins. Galatrox showed optimal hemagglutinating activity at a concentration of 100 μg/ml and this effect was drastically inhibited by lactose, ethylenediaminetetraacetic acid, and heating, which confirmed galatrox's lectin activity. While galatrox failed to induce the same level of paw edema or mast cell degranulation as B. atrox crude venom, galatrox did alter cellular viability, which suggested that galatrox might contribute to venom toxicity by directly inducing cell death. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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