Screening of reducing agents for anaerobic growth of Candida albicans SC5314.

Autor: Rymovicz AU; Xenobiotics Research Unit, Biological and Health Sciences Center, The Pontifical Catholic University of Paraná, Brazil., Souza RD, Gursky LC, Rosa RT, Trevilatto PC, Groppo FC, Rosa EA
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2011 Mar; Vol. 84 (3), pp. 461-6. Date of Electronic Publication: 2011 Jan 21.
DOI: 10.1016/j.mimet.2011.01.020
Abstrakt: This study aimed to evaluate the influence of different redox potentials (Eh) on cell growth, whole-cell protein profile and cell surface hydrophobicity (CSH) of Candida albicans SC5314. The yeast was grown in YNB broth enriched with reducing (158mM sodium sulfite, 4mM sodium sulfite, 2.5mM sodium metabisulfite, 1.3mM 2-mercaptoethanol, 5.5mM thioglycolic acid, and 3.2mM l-cysteine hydrochloride) and oxidizing agents (15mM ammonium persulfate and 80mM potassium ferricyanide) and incubated in normoxic and anoxic atmospheres at 37°C, for 48h. Pre- and post-incubation Eh values were determined and cytoplasm proteins were extracted. Proteins were parted by SDS-PAGE and their profiles were compared. 3.2mM l-cysteine and 1.3mM 2-mercaptoethanol promoted and maintained negative Eh values during incubation. No differences were detected among SDS-PAGE profiles. CSH differences only were observed with 4mM sodium sulfite and 3.2mM l-cysteine. Results showed that 3.2mM l-cysteine is a reducing agent that allows maintenance of negative Eh in both anoxic and normoxic conditions and it seems not to interfere in the global expression of plasmatic proteins.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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