| Autor: |
Chen BA; Department of Hematology, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China. cba8888@hotmail.com, Zhang B, Li CR, Zhou JF, Wu XQ, Gao C, Gao F, Xia GH, Shao ZY, Ding JH, Zhao G, Chen J, Wang J, Song HH, Bao W |
| Jazyk: |
čínština |
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2010 Dec; Vol. 18 (6), pp. 1515-9. |
| Abstrakt: |
This study was aimed to investigate the effect of sodium valproate(VPA) on human myelodysplastic syndrome cell line SKM-1 and its mechanism. The cell proliferation was determined by MTT assay, cell apoptosis was analyzed by flow cytometry. The expressions of c-flipl, c-flips and dlk1 mRNA were detected by RT-PCR. The results showed that VPA could inhibited the growth of SKM-1 cells in dose- and time-dependent manners. The flow cytometric analysis indicated that VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner. The expressions of c-flipl, c-flips and dlk1 mRNA in SKM-1 cell treated with VPA decreased using of VPA. It is concluded that VPA can induce apoptosis and inhibited proliferation of SKM-1 cells. In this process, the decreasing of c-flipl, c-flips and dlk1 mRNA expression may play important roles. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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