Interferon-γ +874 polymorphism in the first intron of the human interferon-γ gene and kidney allograft outcome.

Autor: Crispim JC; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. janacrispim@usp.br, Wastowski IJ, Rassi DM, Mendes-Junior Silva CT, Bassi C, Castelli EC, Costa RS, Saber LT, Silva TG, Donadi EA
Jazyk: angličtina
Zdroj: Transplantation proceedings [Transplant Proc] 2010 Dec; Vol. 42 (10), pp. 4505-8.
DOI: 10.1016/j.transproceed.2010.10.014
Abstrakt: Background: Despite advances in immunosuppressive therapy in the past decade, allograft rejection remains an important cause of kidney graft failure. Cytokines play a major role in the inflammatory and immune responses that mediate allograft outcomes. Several studies have shown that the production of cytokines varies among individuals. These variations are determined by genetic polymorphisms, most commonly within the regulatory region of cytokine genes. The aim of the present study was to assess the effect of allelic variation on acute rejection episodes (ARE) or chronic allograft nephropathy (CAN) after kidney transplantation.
Methods: To determine a possible correlation between the interferon (INF)-γ +874 polymorphism and kidney allograft outcome, we isolated genomic DNA from 74 patients who underwent isolated kidney allografts and were classified into 2 groups--a rejection and a nonrejection group--for comparison with a control group of 163 healthy subjects.
Results: We genotyped INF-γ +874 polymorphisms in all groups. The transplant group showed a significantly increased homozygous genotype T/T (P = .0118) compared with healthy controls. Similarly, considering only patients with CAN, the homozygous genotype T/T (P = .0067) was significantly increased compared with the healthy controls. The rejection group indicated a significant increased homozygous genotype T/T compared with the control group (P = .0061).
Conclusion: Homozygous genotype T/T was associated with increased levels of INF-γ and greater numbers among the rejection and CAN cohorts.
(Copyright © 2010. Published by Elsevier Inc.)
Databáze: MEDLINE
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