| Autor: |
Novikov AA; Institute of Molecular Genetics, USSR Academy of Sciences, Moscow., Borukhov SI, Strongin AYa |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1990 May 31; Vol. 169 (1), pp. 297-301. |
| DOI: |
10.1016/0006-291x(90)91467-7 |
| Abstrakt: |
The plasmid pBINS1, containing the promoter, SD and leader peptide sequences of Bacillus amyloliquefaciens alpha-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of protease-deficient Bacillus subtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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