Increased frequency of anti-retina antibodies in asymptomatic patients with chronic t. gondii infection.
| Autor: | Cursino SR; Protozoology Department, Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil., Costa TB, Yamamoto JH, Meireles LR, Silva MA, Andrade Junior HF |
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| Jazyk: | angličtina |
| Zdroj: | Clinics (Sao Paulo, Brazil) [Clinics (Sao Paulo)] 2010; Vol. 65 (10), pp. 1027-32. |
| DOI: | 10.1590/s1807-59322010001000018 |
| Abstrakt: | Purpose: To search for anti-retina antibodies that serve as markers for eye disease in uveitis. Materials and Methods: Stored sera from patients with uveitis, ocular toxoplasmosis (n = 30) and non-infectious, immune-mediated uveitis (n = 50) and from asymptomatic individuals who were positive (n = 250) and negative (n = 250) for anti-Toxoplasma antibodies were tested. Serum anti-retina IgG was detected by an optimized ELISA using a solid-phase whole human retina extract, bovine S-antigen or interphotoreceptor retinoid-binding protein. Results: Uveitis patients showed a higher mean reactivity to whole human retina extract, interphotoreceptor retinoid-binding protein and S-antigen in comparison to the asymptomatic population. These findings were independent of the uveitis origin and allowed the determination of the lower anti-retina antibody cut-off for the three antigens. Asymptomatic anti-Toxoplasma serum-positive individuals showed a higher frequency of antihuman whole retina extract antibodies in comparison to asymptomatic anti-Toxoplasma serum-negative patients. The bovine S-antigen and interphotoreceptor retinoid-binding protein ELISAs also showed a higher mean reactivity in the uveitis groups compared to the asymptomatic group, but the observed reactivities were lower and overlapped without discrimination. Conclusion: We detected higher levels of anti-retina antibodies in uveitis patients and in a small fraction of asymptomatic patients with chronic toxoplasmosis. The presence of anti-retina antibodies in sera might be a marker of eye disease in asymptomatic patients, especially when whole human retina extract is used in a solid-phase ELISA. |
| Databáze: | MEDLINE |
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