| Autor: |
Gregersen KA; Chemistry Department, University of Washington, Seattle, Washington, USA., Hill ZB, Gadd JC, Fujimoto BS, Maly DJ, Chiu DT |
| Jazyk: |
angličtina |
| Zdroj: |
ACS nano [ACS Nano] 2010 Dec 28; Vol. 4 (12), pp. 7603-11. Date of Electronic Publication: 2010 Nov 30. |
| DOI: |
10.1021/nn102345f |
| Abstrakt: |
This paper describes a method by which molecules that are impermeable to cells are encapsulated in dye-sensitized lipid nanocapsules for delivery into cells via endocytosis. Once inside the cells, the molecules are released from the lipid nanocapsules into the cytoplasm with a single nanosecond pulse from a laser in the far red (645 nm). We demonstrate this method with the intracellular release of the second messenger IP(3) in CHO-M1 cells and report that calcium responses from the cells changed from a sustained increase to a transient spike when the average number of IP(3) released is decreased below 50 molecules per nanocapsule. We also demonstrate the delivery of a 23 kDa O(6)-alkylguanine-DNA alkyltransferase (AGT) fusion protein into Ba/F3 cells to inhibit a key player BCR-ABL in the apoptotic pathway. We show that an average of ∼8 molecules of the inhibitor is sufficient to induce apoptosis in the majority of Ba/F3 cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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