| Autor: |
Bleul R; Institut für Mikrotechnik Mainz GmbH (IMM), Carl-Zeiss-Str. 18-20, 55129 Mainz, Germany., Ritzi-Lehnert M, Höth J, Scharpfenecker N, Frese I, Düchs D, Brunklaus S, Hansen-Hagge TE, Meyer-Almes FJ, Drese KS |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2011 Jan; Vol. 399 (3), pp. 1117-25. Date of Electronic Publication: 2010 Nov 30. |
| DOI: |
10.1007/s00216-010-4446-5 |
| Abstrakt: |
Stopped-flow technology is frequently used to monitor rapid (bio)chemical reactions with high temporal resolution, e.g., in dynamic investigations of enzyme reactions, protein interactions, or molecular transport mechanisms. However, conventional stopped-flow devices are often overly complex, voluminous, or costly. Moreover, excessive amounts of sample are often wasted owing to inefficient designs. To address these shortcomings, we propose a stopped-flow system based on microfluidic design principles. Our simple and cost-efficient approach offers distinct advantages over existing technology. In particular, the use of injection-molded disposable microfluidic chips minimizes required sample volumes and associated costs, simplifies handling, and prevents adverse cross-contamination effects. The cost of the system developed is reduced by an order of magnitude compared with the cost of commercial systems. The system contains a high-precision valve system for fluid control and features automated data acquisition capability with high temporal resolution. Analyses with two well-established reaction kinetics yielded a dead time of approximately 8-9 ms. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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