| Autor: |
Smith DJ; Department of Microbiology, University of Bristol, UK., Burnham MK, Bull JH, Hodgson JE, Ward JM, Browne P, Brown J, Barton B, Earl AJ, Turner G |
| Jazyk: |
angličtina |
| Zdroj: |
The EMBO journal [EMBO J] 1990 Mar; Vol. 9 (3), pp. 741-7. |
| DOI: |
10.1002/j.1460-2075.1990.tb08168.x |
| Abstrakt: |
A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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