[Detection of parvovirus B19 markers in blood samples of donors].

Autor:	Filatova EV, Zubkova NV, Novikova NA, Golitsina LN, Kuznetsov KV
Jazyk:	ruština
Zdroj:	Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2010 Sep-Oct (5), pp. 67-70.
Abstrakt:	Aim: To assess detection rate of parvovirus B19 markers in population of donors in one of the regions of Russian Federation. Materials and Methods: Screening of blood samples from 1000 donors for parvovirus B19 DNA was performed by real-time polymerase chain reaction. Levels of specific antibodies to parvovirus B19 were measured by quantitative enzyme immunoassay (R-Biopharm AG, Germany). Results: Parvovirus B19 DNA was detected in 10 samples (1%) with viral load ranging from 1.0 x 10(3) to 1.0 x 10(6) genome equivalents per 1 ml. All DNA-positive samples contained IgG, and one sample contained IgM also. IgG were detected in 29.7% of DNA-negative samples. In these samples specific IgM were not detected. Mean level of IgG in DNA-negative and DNA-positive groups was 27.52 IU/ml [95% CI (19.6 -35.4) IU/ml] and 107.30 IU/ml [95% CI (55.7 - 158.9) IU/ml] respectively. Level of IgG in DNA-negative group was significantly lower than in the DNA-positive (p < 0.0001). Conclusion: Results of the study provide new and important information for making rational decision about screening of donors' blood for parvovirus B19 markers.
Databáze:	MEDLINE
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