| Autor: |
Melgunov VI; Department of Molecular Biology, School of Biology, M.V. Lomonosov State University, Moscow, USSR., Mamedova NA, Akimova EI, Adzhimolaev TA |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1990 Jan 15; Vol. 260 (1), pp. 79-82. |
| DOI: |
10.1016/0014-5793(90)80070-y |
| Abstrakt: |
By using extraction in the presence of Ca2+ and Triton X-100 and then in the presence of EGTA without detergent, a set of Ca2+-dependent phospholipid binding proteins has been identified in the membranes of transverse tubules (T-tubules) and sarcoplasmic reticulum (SR), isolated from rabbit skeletal muscles. Longitudinal SR, junctional SR and T-tubule membranes yielded about 9, 14 and 3.3 micrograms of EGTA-soluble proteins per 1 mg of membrane protein, respectively. In the presence of 1 mM CaCl2, 68 and 33 kDa proteins of T-tubules and junctional SR as well as 30 kDa protein of T-tubules were shown to bind to liposomes made of 1:1 w/w mixtures of (i) phosphatidylcholine and (ii) phosphatidylserine, phosphatidic acid, or phosphatidyl ethanolamine. In the presence of EGTA, the above-mentioned proteins were mostly found in the supernatants. Binding of the proteins with liposomes consisting of pure phosphatidylcholine was negligible. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text