Multicomponent Moraxella catarrhalis outer membrane vesicles induce an inflammatory response and are internalized by human epithelial cells.

Autor: Schaar V; Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Skåne University Hospital, Sweden., de Vries SP, Perez Vidakovics ML, Bootsma HJ, Larsson L, Hermans PW, Bjartell A, Mörgelin M, Riesbeck K
Jazyk: angličtina
Zdroj: Cellular microbiology [Cell Microbiol] 2011 Mar; Vol. 13 (3), pp. 432-49. Date of Electronic Publication: 2010 Nov 24.
DOI: 10.1111/j.1462-5822.2010.01546.x
Abstrakt: Moraxella catarrhalis is an emerging human respiratory pathogen in patients with chronic obstructive pulmonary disease (COPD) and in children with acute otitis media. The specific secretion machinery known as outer membrane vesicles (OMVs) is a mechanism by which Gram-negative pathogens interact with host cells during infection. We identified 57 proteins in M. catarrhalis OMVs using a proteomics approach combining two-dimensional SDS-PAGE and MALDI-TOF mass spectrometry analysis. The OMVs contained known surface proteins such as ubiquitous surface proteins (Usp) A1/A2, and Moraxella IgD-binding protein (MID). Most of the proteins are adhesins/virulence factors triggering the immune response, but also aid bacteria to evade the host defence. FITC-stained OMVs bound to lipid raft domains in alveolar epithelial cells and were internalized after interaction with Toll-like receptor 2 (TLR2), suggesting a delivery to the host tissue of a large and complex group of OMV-attributed proteins. Interestingly, OMVs modulated the pro-inflammatory response in epithelial cells, and UspA1-bearing OMVs were found to specifically downregulate the reaction. When mice were exposed to OMVs, a pulmonary inflammation was clearly seen. Our findings indicate that Moraxella OMVs are highly biologically active, transport main bacterial virulence factors and may modulate the epithelial pro-inflammatory response.
(© 2010 Blackwell Publishing Ltd.)
Databáze: MEDLINE
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