| Autor: |
Misuno NI; Byelorussian Institute of Hematology and Blood Transfusion, Minsk, USSR., Osipovich OA, Sudarikov AB, Idelson GL, Kolesnikova TS, Panyutich AV, Voitenok NN |
| Jazyk: |
angličtina |
| Zdroj: |
Cytokine [Cytokine] 1990 Nov; Vol. 2 (6), pp. 464-9. |
| DOI: |
10.1016/1043-4666(90)90056-y |
| Abstrakt: |
The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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