TMPRSS2-ERG gene fusion in small cell carcinoma of the prostate.

Autor: Guo CC; Department of Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030-4009, USA., Dancer JY, Wang Y, Aparicio A, Navone NM, Troncoso P, Czerniak BA
Jazyk: angličtina
Zdroj: Human pathology [Hum Pathol] 2011 Jan; Vol. 42 (1), pp. 11-7. Date of Electronic Publication: 2010 Oct 30.
DOI: 10.1016/j.humpath.2010.05.026
Abstrakt: Recent studies have shown that most prostate cancers carry the TMPRSS2-ERG gene fusion. Here we evaluated the TMPRSS2-ERG gene fusion in small cell carcinoma of the prostate (n = 12) in comparison with small cell carcinoma of the urinary bladder (n = 12) and lung (n = 11). Fluorescence in situ hybridization demonstrated rearrangement of the ERG gene in 8 cases of prostatic small cell carcinoma (67%), and the rearrangement was associated with deletion of the 5' ERG gene in 7 cases, but rearrangement of the ERG gene was not present in any small cell carcinoma of the urinary bladder or lung. Next we evaluated the TMPRSS2-ERG gene fusion in nude mouse xenografts that were derived from 2 prostatic small cell carcinomas carrying the TMPRSS2-ERG gene fusion. Two transcripts encoded by the TMPRSS2-ERG gene fusion were detected by reverse transcriptase polymerase chain reaction, and DNA sequencing demonstrated that the 2 transcripts were composed of fusions of exon 1 of the TMPRSS2 gene to exon 4 or 5 of the ERG gene. Our study demonstrates the specific presence of TMPRSS2-ERG gene fusion in prostatic small cell carcinoma, which may be helpful in distinguishing small cell carcinoma of prostatic origin from nonprostatic origins. The high prevalence of the TMPRSS2-ERG gene fusion in prostatic small cell carcinoma as well as adenocarcinoma implies that small cell carcinoma may share a common pathogenic pathway with adenocarcinoma in the prostate.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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