Fetal calf serum enhances in vitro production of Bos taurus indicus embryos.

Autor: Leivas FG; Embryolab-Laboratório de Embriologia Animal, Departamento de Clínica de Grandes Animais, Centro de Ciências Rurais, Universidade Federal Santa Maria, 97.105-900 Santa Maria RS, Brazil., Brum DS, Fialho SS, Saliba WP, Alvim MT, Bernardi ML, Rubin MI, Silva CA
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2011 Feb; Vol. 75 (3), pp. 429-33. Date of Electronic Publication: 2010 Oct 18.
DOI: 10.1016/j.theriogenology.2010.08.017
Abstrakt: The objective of this study was to determine the effect of fetal calf serum (FCS) on the quality of in vitro produced bovine embryos. Cumulus oocyte-complexes (COCs, n = 2 449) recovered by ovum pick-up from Bos taurus indicus donors were randomly assigned to experimental groups. Sperm selected by Percoll gradient was used for in vitro fertilization (insemination = Day 0). In Experiment 1 (n = 1 745 COCs), zygotes were cultured in vitro in Synthetic Oviduct Fluid + 4 mg/mL of bovine serum albumin (BSA), or BSA + 2% FCS (BSA+FCS). In Experiment 2 (n = 704 COCs), the COCs were cultured in SOF + BSA, BSA + 2% FCS, or BSA + 2% FCS on D4 (BSA + FCSD4). In Experiment 1, blastocyst yield (51%) and Quality I blastocysts (41%) at Day 7 were higher (P < 0.05) in the BSA + FCS treatment than in BSA (42 and 30%, respectively). In Experiment 2, blastocyst yield was higher (P < 0.05) in the BSA+FCS (47%) treatment. Quality I blastocyst yield was higher (P < 0.05) for BSA + FCS (34%) and BSA+FCSD4 (32%) compared to the BSA treatment (20%). A total of 820 embryos were transferred, with no significant differences among groups in pregnancy rates. In conclusion, in vitro culture in SOFaaci + BSA + FCS enhanced blastocyst yield and Quality I blastocysts; adding FCS to the culture medium increased the efficiency of IVP of bovine embryos.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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