| Autor: |
Billedeau SM; Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079., Holder CL, Getek TA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1990 Dec 14; Vol. 534, pp. 151-9. |
| DOI: |
10.1016/s0378-4347(00)82157-x |
| Abstrakt: |
The electrochemical behavior of the over-the-counter antihistamine drug pyrilamine and its N-oxide analogue, have been studied by several voltammetric methods. Cyclic voltammograms of pyrilamine maleate in 0.1 M ammonium acetate at pH 7.0 indicated a quasi-reversible electrode process by observing a wave at + 0.85 V and + 1.30 V in the initial anodic sweep followed by a wave at - 1.30 V versus Ag/AgCl. Differential pulse and hydrodynamic voltammetry of pyrilamine and the N-oxide were examined to determine oxidation potentials for use in high-performance liquid chromatography with electrochemical detection (HPLC-ED). Differentiation between pyrilamine and its N-oxide was achieved in HPLC-ED analyses at a detection potential of + 0.7 V and + 0.9 V versus Ag/AgCl with tandem ultraviolet detection at 254 nm. Utility of the HPLC-ED method was demonstrated by the analysis of pyrilamine and the N-oxide in microbial biotransformation samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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