| Autor: |
Vieira HL; Instituto de Biologia Experimental e Tecnologia/Instituto de Tecnologia Química e Biológica IBET/ITQB -UNL, Apartado 12, 2781-901, Oeiras, Portugal., Pereira AC, Peixoto CC, Moraes RH, Alves PM, Mendonça RZ |
| Jazyk: |
angličtina |
| Zdroj: |
Cytotechnology [Cytotechnology] 2010 Dec; Vol. 62 (6), pp. 547-55. Date of Electronic Publication: 2010 Oct 10. |
| DOI: |
10.1007/s10616-010-9305-x |
| Abstrakt: |
Apoptosis is a major problem in animal cell culture during production of biopharmaceuticals, such as recombinant proteins or viral particles. In the present work baculovirus-insect cell expression system (BEVS/IC) is used as model to produce rotavirus like-particles, composed by three layers of three different viral proteins (VP2, VP6 and VP7). In this model baculovirus infection also induces host cell death. Herein a new strategy to enhance cell life span and to increase recombinant rotavirus protein production of BEVS/IC system was developed. This strategy relies on hemolymph from Lonomia oblique (total extracts or a semi-purified fraction) medium supplementation. The total extract and a purified fraction from hemolymph of Lonomia obliqua were able to protect Sf-9 cell culture against apoptosis triggered by oxidative stress (using the pro-oxidant agents tert butylhydroperoxide and hydrogen peroxide) and by baculovirus infection. Furthermore, hemolymph enhance final recombinant protein production, as it was observed by the increased amounts of VP6 and VP7, which were measured by the semi-quantitative western blot method. In conclusion, hemolymph medium supplementation can be a promising strategy to improve cell viability and productivity of recombinant protein in BEVS/IC system. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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