| Autor: |
Buccigrossi V; Departments of Paediatrics, University of Naples, 'Federico II,' Naples 80131, Italy., Armellino C, Ruberto E, Barone MV, Marco GD, Esposito C, Guarino A |
| Jazyk: |
angličtina |
| Zdroj: |
Pediatric research [Pediatr Res] 2011 Jan; Vol. 69 (1), pp. 17-22. |
| DOI: |
10.1203/PDR.0b013e3181ff61ba |
| Abstrakt: |
Guanylin receptor guanylate cyclase (GC-C) peaks in neonatal intestine and is involved in either enterocyte proliferation or chloride secretion. The latter is more potent when GC-C activator guanylin, or its analog Escherichia coli heat-stable enterotoxin (ST), is added to the mucosal rather than serosal side of intestinal monolayers. By using Ussing chambers, we investigated transepithelial ion transport and enterocyte proliferation and their mechanisms in response to the addition of guanylin or ST to the mucosal or serosal side of Caco-2 monolayers and in ileal specimens from neonates. GC-C activation showed a polar pattern of the effects. GC-C mucosal activation resulted in a potent cGMP-chloride secretion activation and in a marginal enterocyte proliferation. Conversely, serosal GC-C activation induced a potent enterocyte proliferation, through MAP kinase ERK 1/2. Finally, the inhibition of ERK1/2 enhanced the Isc increase in response to serosal but not to mucosal ST stimulation, indicating that ERK1/2 also acts as a brake of chloride secretion. These data suggest that the guanylin/GC-C system plays a key role in early postnatal intestinal adaptation exploiting the polar structure of enterocyte. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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