Investigation of MADS domain transcription factor dynamics in the floral meristem.

Autor: Urbanus SL; Plant Research International, Droevendaalsesteeg, The Netherlands., Dinh QD, Angenent GC, Immink RG
Jazyk: angličtina
Zdroj: Plant signaling & behavior [Plant Signal Behav] 2010 Oct; Vol. 5 (10), pp. 1260-2. Date of Electronic Publication: 2010 Oct 01.
DOI: 10.4161/psb.5.10.12949
Abstrakt: To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it indicated a possible non-cell-autonomous role for the B-type proteins APETALA3 and PISTILLATA, and the E-type protein SEPALLATA3, through lateral intercellular movement in the floral meristem. In this addendum we compare some of the available fluorescent protein-based technologies for the investigation of transcription factor movements and dynamics.
(© 2010 Landes Bioscience)
Databáze: MEDLINE