Screening of platelets for bacterial contamination at the Welsh Blood Service.
| Autor: | Pearce S; Welsh Blood Service, Laboratory Services, Pontyclun, UK. stephen.pearce@wales.nhs.uk, Rowe GP, Field SP |
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| Jazyk: | angličtina |
| Zdroj: | Transfusion medicine (Oxford, England) [Transfus Med] 2011 Feb; Vol. 21 (1), pp. 25-32. Date of Electronic Publication: 2010 Sep 21. |
| DOI: | 10.1111/j.1365-3148.2010.01037.x |
| Abstrakt: | Background and Objective: This report details the results of the implementation of a bacterial screening system at the Welsh Blood Service and provides an estimate of the levels of bacterial contamination at the time of sampling. Materials and Methods: Apheresis (Caridian BCT) and buffy coat-derived pooled platelet components were sampled on day 1 for bacterial contamination and the sample was monitored throughout the lifespan of the platelet component. Unused platelet components were re-tested to determine the effectiveness of the screening. Results from the BacT/ALERT are uploaded to the in-house Blood Establishment Computer System (BECS) every 12 min. Positive alerts are automatically sent to staff, facilitating a timely intervention. Results: Between February 2003 and March 2010 the screening system tested 54 828 platelets and detected 257 (1 in 213) initial positives of which 35 (1 in 1567, 0·06%) were confirmed [95% confidence interval (CI), 0·04-0·08%]. Additionally, screening of 6438 unused platelet components detected another 6 (1 in 1073, 0·09%) confirmed positives not detected during initial testing (95% CI, 0·02-0·16%). Analysis of the data suggests that on day 1 the number of bacteria in such platelet component packs was between 5 and 62 cfus total. Day 1 culture has a sensitivity of 40%. Conclusions: The bacterial screening system has removed a significant number, but not all bacterially contaminated platelet components from the supply. The sample volume is an important factor in sensitivity due to the low number of bacteria in a platelet component pack on day 1. An effective notification and recall system is a critical part of the bacterial screening system. (© 2010 The Authors. Transfusion Medicine © 2010 British Blood Transfusion Society.) |
| Databáze: | MEDLINE |
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