| Autor: |
Gaggelli E; Department of Chemistry, University of Siena, Via Aldo Moro, 53100, Siena, Italy., Gaggelli N, Molteni E, Valensin G, Balenci D, Wrońska M, Szczepanik W, Nagaj J, Skała J, Jeżowska-Bojczuk M |
| Jazyk: |
angličtina |
| Zdroj: |
Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2010 Nov 07; Vol. 39 (41), pp. 9830-7. Date of Electronic Publication: 2010 Sep 14. |
| DOI: |
10.1039/c0dt00458h |
| Abstrakt: |
The aminoglycosidic antibiotic hygromycin B presents a peculiar chemical structure, characterized by two sugar rings joined via a spiro connection. The Cu(ii) complex of hygromycin B in water solution was characterized by (1)H-NMR, UV-Vis, EPR and CD spectroscopy, combined with potentiometric measurements. The spin-lattice relaxation enhancements were interpreted by the Solomon-Bloembergen-Morgan theory, allowing us to calculate copper-proton distances that were used to build a model of the complex by molecular mechanics and dynamics calculations. The fidelity of the proposed molecular model was checked by ROESY maps. Moreover DNA damage by the Cu(ii)-hygromycin B system was also investigated, showing single and double strand scissions exerted by the complex at concentrations in the range 1-5 mM. Addition of either hydrogen peroxide or ascorbic acid to each sample resulted in the shift of the cleavage potency towards lower concentrations of the complex. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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