Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.
| Autor: | Simões Sde M; Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10065, USA., Blankenship JT, Weitz O, Farrell DL, Tamada M, Fernandez-Gonzalez R, Zallen JA |
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| Jazyk: | angličtina |
| Zdroj: | Developmental cell [Dev Cell] 2010 Sep 14; Vol. 19 (3), pp. 377-88. |
| DOI: | 10.1016/j.devcel.2010.08.011 |
| Abstrakt: | Cell rearrangements shape the Drosophila embryo via spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of the Baz domain, and activated Rho-kinase is sufficient to exclude Baz from the cortex. The planar polarized distribution of Baz requires its C-terminal domain. Rho-kinase can phosphorylate this domain and inhibit its interaction with phosphoinositide membrane lipids, suggesting a mechanism by which Rho-kinase could regulate Baz association with the cell cortex. These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains. (Copyright © 2010 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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