| Abstrakt: |
Sulfate-reducing bacteria and their heterotrophic satellites have been isolated from the biofilm formed on steel by sulfidogenic corrosive microbial community. Bacteria were characterized according to phenotypical features and investigated by the methods of molecular-genetic analysis. In accordance with the phenotypical features the studied strain of sulfate-reducing bacteria were related to Desulfovibrio genus, Gram-positive strains of heterotrophic satellites were related to Bacillus genus, B. firmus and B. subtilis species. Gram-negative strains, as to their physiological-biochemical characteristics were related to Pseudomonas genus, P. aeruginosa species, Stenotrophomonas genus, S. maltophilia species, and Aeromonas genus, A. hydrophila/caviae species. Taxonomic position of certain representatives of the community is confirmed by the molecular-genetic methods. A comparative analysis of the sequencing results has evidenced for the identity of sequences of 16S rRNA of the studied bacteria with analogous sequence of strains from the GenBank database. Nucleotide sequence of strain 27 has a 99% homology with the sequence of Pseudomonas aeruginosa, strain 36 deposited at GenBank to nucleotide sequence of Bacillus subtilis. As to sulfate-reducing bacteria, only their belonging to Desulfovibrio genus has been confirmed. Thus the results of sequence-analysis of 16S rRNA genes are in agreement with the data obtained from studying the phenotypical features. |
