Enhancement of magnetotactic bacterial yield in a modified MSGM medium without alteration of magnetosomes properties.

Autor: Kundu S; School of Basic Medical Sciences, Department of Physics, University of Pune, Pune 411 007, India. srikanya19@gmail.com, Kulkarni GR
Jazyk: angličtina
Zdroj: Indian journal of experimental biology [Indian J Exp Biol] 2010 May; Vol. 48 (5), pp. 518-23.
Abstrakt: Magnetotactic bacteria (MTB), Magnetospirillum magnetotacticum (MS-1) were successfully grown in modified magnetic spirillum growth medium (MSGM) at normal laboratory environment. About five-time increase in the bacterial yield was achieved in the modified MSGM medium without compromising their magnetosomes properties. Transmission electron and scanning electron microscopy (TEM & SEM) were used for morphological study of MTB. Energy dispersive analysis of X-rays (EDAX) and vibrating sample magnetometer (VSM) techniques, respectively, were used to elucidate the phase and magnetization in the bacterially synthesized magnetosomes. These studies were important to cross-check the morphology of magnetosomes, as the formation of magnetosomes was highly sensitive to environmental conditions.
Databáze: MEDLINE