| Autor: |
Reed J; Department of Chemistry and Biochemistry, UCLA, 607 Charles Young Drive East, Los Angeles, CA 90095, USA., Frank M, Troke JJ, Schmit J, Han S, Teitell MA, Gimzewski JK |
| Jazyk: |
angličtina |
| Zdroj: |
Nanotechnology [Nanotechnology] 2008 Jun 11; Vol. 19 (23), pp. 235101. |
| DOI: |
10.1088/0957-4484/19/23/235101 |
| Abstrakt: |
The dynamic nanomechanical properties of a large number of cells (up to hundreds), measured in parallel with high throughput, are reported. Using NIH 3T3 and HEK 293T fibroblasts and actin depolymerizing drugs, we use a novel nanotechnology to quantify the local viscoelastic properties with applied forces of 20 pN-20 nN, a spatial resolution of <20 nm, and a mechanical dynamic range of several Pa up to ~200 kPa. Our approach utilizes imaging interferometry in combination with reflective, magnetic probes attached to cells. These results indicate that mechanical imaging interferometry is a sensitive and scalable technology for measuring the nanomechanical properties of large arrays of live cells in fluid. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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