| Autor: |
Cookman GR; Department of Pharmacology, University College, Belfield, Dublin 4, Ireland., Regan CM |
| Jazyk: |
angličtina |
| Zdroj: |
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 1991; Vol. 5 (2), pp. 127-32. |
| DOI: |
10.1016/0887-2333(91)90032-9 |
| Abstrakt: |
Inorganic lead inhibited proliferation of the C6 glial cell line with an IC(50) value of 5 x 10(-5)m-lead chloride. Cells released from a 48-hr exposure to 10(-4)m-lead chloride did not show a coherent [(3)H]thymidine incorporation on entering the S phase of the cell cycle, which suggested that they lacked synchrony. Lead did not appear to compromise DNA biosynthetic capability, since synchronous cells, obtained by mitotic selection, coherently entered S phase after a 14-hr exposure to 10(-4)m-PbCl(2). Cells maintained for 48 hr in 10(-4)m-PbCl(2) exhibited morphologies typical of an asynchronous population; however, 1 hr after release, selection of mitotic figures indicated that 35% of these cells had entered M phase. We conclude that inorganic lead has no specific antiproliferative site of action but may contribute to a lengthening of the cell cycle, the most pronounced effect being in late G2. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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