Fine-scale spatial variability in anatoxin-a and homoanatoxin-a concentrations in benthic cyanobacterial mats: implication for monitoring and management.

Autor: Wood SA; Cawthron Institute, Nelson, New Zealand. susie.wood@cawthron.org.nz, Heath MW, Kuhajek J, Ryan KG
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2010 Dec; Vol. 109 (6), pp. 2011-8.
DOI: 10.1111/j.1365-2672.2010.04831.x
Abstrakt: Aims: The purpose of this study was to determine the variability in anatoxin-a (ATX) and homoanatoxin-a (HTX) concentrations in benthic cyanobacterial mats within sampling sites and to assess the applicability of using a PCR-based approach to determine ATX- and HTX-production potential.
Methods and Results: ATX and HTX variability was investigated by collecting 15 samples from 10 × 10 m grids in seven rivers. ATX and HTX concentrations were determined using liquid chromatography-mass spectrometry (LC-MS). Samples from two sites contained no ATX or HTX and at one site ATX and HTX were detected in all samples. At four sites, both toxic and nontoxic samples co-occurred and these samples were sometimes spaced less than 1 m apart. PCR amplification of a region of a polyketide synthase (ks2, putatively involved in the biosynthetic pathway of ATX and HTX) successfully distinguished ATX-and-HTX- and non-ATX-and-HTX-producing cultured Phormidium strains. Results from environmental samples were more variable, and the results were in congruence with the LC-MS data in only 58% of samples.
Conclusions: Fine-scale spatial variability in ATX and HTX concentrations occurs among benthic cyanobacterial mats.
Significance and Impact of the Study: Multiple benthic cyanobacterial mat samples must be collected at a sampling site to provide an accurate assessment of ATX and HTX concentrations at that location. The PCR-based technique offers the potential to be a useful early warning technique.
(© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology.)
Databáze: MEDLINE
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