Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.

Autor: Tanomsing N; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Imwong M, Theppabutr S, Pukrittayakamee S, Day NP, White NJ, Snounou G
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2010 Oct; Vol. 48 (10), pp. 3735-7. Date of Electronic Publication: 2010 Aug 11.
DOI: 10.1128/JCM.00898-10
Abstrakt: A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.
Databáze: MEDLINE