The effect of exopolysaccharide-producing probiotic strains on gut oxidative damage in experimental colitis.

Autor: Sengül N; Department of General Surgery, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Emek Mah. 8. Cad 75.Sok No: 53/2, 06510, Emek, Ankara, Turkey. nerimansengul@hotmail.com, Işık S, Aslım B, Uçar G, Demirbağ AE
Jazyk: angličtina
Zdroj: Digestive diseases and sciences [Dig Dis Sci] 2011 Mar; Vol. 56 (3), pp. 707-14. Date of Electronic Publication: 2010 Aug 04.
DOI: 10.1007/s10620-010-1362-7
Abstrakt: Background: Oxidative stress plays a role in disease initiation and progression in inflammatory bowel disease (IBD) and manipulation of this pathway may attenuate disease progress. In this study, the effect of exopolysaccharide (EPS)-producing probiotic bacteria on gut oxidative damage was evaluated in a rat model of experimental colitis.
Methods: Colitis was induced by intracolonic administration of acetic acid. Rats were treated daily with two probiotic strains, L. delbrueckii subsp. bulgaricus B3 strain (EPS of 211 mg/l; high-EPS group) or L. delbrueckii subsp. bulgaricus A13 strain (EPS of 27 mg/l; low-EPS group), which were given directly into the stomach. Non-colitis-fed control and preventative groups were only treated with the high-EPS producing strain. Antioxidant enzyme activities (superoxide dismutase, catalase, total glutathione, reduced glutathione, glutathione disulfide) and lipid peroxidation were measured in colonic tissue samples after a treatment period of 7 days.
Results: Significant oxidative damage was associated with a higher level of malondialdehyde (MDA) activity and reduced antioxidant enzyme activities in the colitis model group. All antioxidant enzyme activities were higher in both probiotic-treated groups compared with those of the colitis model group (P < 0.001). Lipid peroxidation was significantly ameliorated in both probiotic groups. The improvement of oxidative stress parameters was significantly more in the high-EPS group than in the low-EPS group (P < 0.001).
Conclusions: EPS-producing probiotic bacteria significantly attenuate oxidative stress in experimental colitis. Increased EPS production gives rise to a better probiotic function. These results suggest that EPS molecules could revaluate probiotic strains and exert their beneficial effects on the host and this may have a therapeutic potential.
Databáze: MEDLINE
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