| Autor: |
Mantur B; Department of Microbiology, Belgaum Institute of Medical Sciences, Belgaum, Karnataka, India. drbgmantur@rediffmail.com, Parande A, Amarnath S, Patil G, Walvekar R, Desai A, Parande M, Shinde R, Chandrashekar M, Patil S |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2010 Aug; Vol. 83 (2), pp. 314-8. |
| DOI: |
10.4269/ajtmh.2010.09-0790 |
| Abstrakt: |
The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT), and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2-ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; P < 0.001) patients with brucellosis, including 31 culture-confirmed cases. The sensitivity and specificity of ELISA were 100% and 71.31%, respectively. Because they were confirmed by ELISA, the diagnosis could never be excluded with SAT in 33 cases. ELISA has been found to be more sensitive in acute (28% higher sensitivity; P < 0.02) and chronic (55% higher sensitivity; P < 0.01) cases. For accurate diagnosis in suspected brucellosis cases detection, we recommend both ELISA IgM and IgG tests. ELISA IgG and 2-ME tests seem to be promising tools in judging prognosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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