| Autor: |
Petrov AM; Kazan State Medical University, 49 Butlerov Street, 420012, Kazan, Russia. fysio@rambler.ru, Kasimov MR, Giniatullin AR, Tarakanova OI, Zefirov AL |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroscience and behavioral physiology [Neurosci Behav Physiol] 2010 Oct; Vol. 40 (8), pp. 894-901. Date of Electronic Publication: 2010 Aug 03. |
| DOI: |
10.1007/s11055-010-9338-9 |
| Abstrakt: |
Experiments on frog neuromuscular preparations using electrophysiological (two-electrode voltage clamping) and optical (with the fluorescent endocytic stain FM1-43) methods were performed to study the importance of membrane cholesterol in the exo- and endocytic cycle of synaptic vesicles (SV) in motor nerve endings in conditions of prolonged rhythmic stimulation of the motor nerve (20 impulses/sec, 3 min). Extraction of cholesterol from the superficial plasma membranes using methyl-beta-cyclodextrin (1 mM) led to marked changes in SV recycling. There was weakening of SV exocytosis and suppression of processes leading to the recovery of SV populations with rapid readiness to release neurotransmitter. When cholesterol was leached from the outer membranes and the membranes of SV undergoing recycling, these effects were supplemented by impairments to SV endocytosis and recycling. Thus, plasma membrane cholesterol plays a key role in the processes of exocytosis, while the efficiency of endocytosis depends on cholesterol in SV membranes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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