Synthesis and properties of triplex-forming oligonucleotides containing 2'-O-(2-methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine.
| Autor: | Lou C; School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK., Xiao Q, Brennan L, Light ME, Vergara-Irigaray N, Atkinson EM, Holden-Dye LM, Fox KR, Brown T |
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| Jazyk: | angličtina |
| Zdroj: | Bioorganic & medicinal chemistry [Bioorg Med Chem] 2010 Sep 01; Vol. 18 (17), pp. 6389-97. Date of Electronic Publication: 2010 Jul 30. |
| DOI: | 10.1016/j.bmc.2010.07.005 |
| Abstrakt: | 2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases. (Copyright 2010 Elsevier Ltd. All rights reserved.) |
| Databáze: | MEDLINE |
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