Manzamine A as a novel inhibitor of herpes simplex virus type-1 replication in cultured corneal cells.
| Autor: | Palem JR; Department of Basic Pharmaceutical Sciences, College of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71201, USA., Bedadala GR, El Sayed KA, Hsia SC |
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| Jazyk: | angličtina |
| Zdroj: | Planta medica [Planta Med] 2011 Jan; Vol. 77 (1), pp. 46-51. Date of Electronic Publication: 2010 Jul 19. |
| DOI: | 10.1055/s-0030-1250093 |
| Abstrakt: | This study investigated the putative inhibitory effect of manzamine A on HSV-1 infection. Our results indicated that manzamine A effectively inhibited viral replication and infection in the cell line SIRC, a corneal cell line, at 1 µM. The existing anti-HSV-1 drug acyclovir was analyzed and showed a comparable activity at 50 µM. Plaque assays demonstrated that manzamine A reduced the release of infectious virus by 10 (11)-fold. RTPCR assays indicated that HSV-1 virion host shutoff (vhs) activity and ICP0 transcription were decreased by manzamine A treatment. These results bode well for the development of manzamines as potential leads to reduce viral infection in corneal cells and to prevent HSV-1-induced eye infections such as keratitis. (© Georg Thieme Verlag KG Stuttgart · New York.) |
| Databáze: | MEDLINE |
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